Custom reagents
From proteins to plasmids, we deliver the quality and consistency your discovery needs demand.
High-quality reagents are crucial for maintaining reliability and consistency in process outcomes and product characterization. These reagents must exhibit:
- Batch-to-batch consistency
- Uniform composition
- High purity
- Desired activity
Premium reagents facilitate accurate measurements, enhance assay sensitivity and improve signal-to-noise ratios. They are essential throughout the lifecycle of therapeutic drug candidates, from early discovery to development and commercialization.
We provide faster, flexible and cost-effective solutions to supply reagents for clients’ needs. Our streamlined reagent service workflows ensure a reliable supply of high-quality, well-characterized reagents, offering tailored solutions for drug discovery, drug development and various research endeavors.
Protein and antibody reagents
From gene to purified protein, we offer an integrated approach to generate well-characterized, high-quality, research-grade recombinant proteins and antibodies at mg-to-gram scale with rapid turnaround (as early as 3-4 weeks). Our multi-host expression platforms include:
- Mammalian: CHO and HEK
- Microbial: Escherichia coli
Our state-of-the-art analytical capabilities support comprehensive protein characterization, seamlessly integrated with our manufacturing processes. This integration facilitates scale-up and GMP requirements when needed.
Synthetic peptides
Our offering encompasses both solid-state and liquid-phase synthesis platforms for generating synthetic peptides for research and therapeutic applications. Our capabilities include:
- Synthesis of complex peptides (up to 40-50 amino acids)
- Quantities ranging from milligrams to multi-grams
- Various modifications (cyclic peptides, disulfide bridges, branched peptides, PEGylation)
- Additional modifications (biotinylation, acetylation, amidation)
- Green chemistry approaches
We ensure high purity and extensive characterization, including impurity profiles. Our focus on sustainability has led to the development of green chemistry workflows for peptide synthesis, reducing waste and lowering the carbon footprint.
Advanced modalities
We have developed streamlined workflows to supply a range of advanced modality reagents, including AAV plasmids, stable xRNA and purified, well-characterized exosomes from various cell sources. These optimized processes have expanded our impact on biopharmaceutical research, particularly in the areas of mRNA, cell therapy and gene therapy development. Our comprehensive offerings in advanced modalities include:
Adeno-associated virus (AAV) is a versatile viral vector used for gene therapy delivery. AAVs can be engineered to specifically deliver DNA to target cells, making them ideal for various therapeutic applications. We offer in vitro POC for major AAV serotypes and an off-the-shelf AAV plasmid kit (AAVEnzene®) for producing rAAV particles. For gene therapies, we utilize recombinant AAV (rAAV), which lacks viral DNA, providing a safe and effective delivery system for clinical and preclinical applications.
Our off-the-shelf AAV plasmid kit, AAVENZENE®, includes essential tools for producing rAAV particles:
- AAV RepCap plasmid
- AAV helper plasmid
- AAV expression plasmid for cloning the gene of interest (GOI)
These plasmids encode key components for rAAV particle preparation. Using the three-plasmid (triple) transfection method, components are expressed and rAAV particles are produced. A positive control with yellow fluorescent protein (YFP) as the GOI is also provided.
Enzene offers comprehensive services for high-quality RNA production, including mRNA, miRNA, siRNA, shRNA and antisense RNA. Our services cover the entire RNA synthesis process, from design and optimization to large-scale production and purification. Robust quality control measures ensure accuracy, purity and stability of RNA products for applications ranging from basic research to therapeutic development.
Leveraging advancements in cellular components for therapeutic and diagnostic purposes, we have optimized workflows to extract and characterize exosomes from primary and engineered cells. Using state-of-the-art technologies like size-exclusion chromatography and immunoaffinity capture, Enzene provides high-quality reagent-grade exosome preparations for diagnostic research, therapeutic development and personalized medicine.
AAV
Off-the-shelf AAV plasmids toolkit (Helper, RepCap2, PepCap4, RepCap5) with tested functionality. Rapid custom plasmid manufacturing at R&D and GMP scale with functionality testing
RNA
Design and synthesis of premium quality and stable RNA. Well-characterized RNA: mRNA, siRNA, shRNA, miRNA, antisense RNA
Exosomes
Highly pure exosomes from primary cells and established cell lines. High-end analytical panel for characterization of exosomes
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Fueled by our continuous innovation and armed with EnzeneX™, our fully-integrated CDMO solutions and our biosimilars pipeline are designed to help bring your biologics innovations to life.